La Biblia Alfonsina had a profound impact on the development of the Spanish language. The translation helped to standardize Spanish vocabulary and grammar, contributing to the emergence of a distinct literary language. The text also showcases the translators' skill in rendering complex Latin concepts into vernacular Spanish, paving the way for future literary works. Furthermore, the Biblia Alfonsina influenced the development of Spanish literature, inspiring later writers, such as Don Quixote author Miguel de Cervantes.